The relatively long regions of homology required for some Gibson assembly reactions will be more prone to secondary structure, which potentially interfere with successful outcomes.However, related Gibson assembly reagents, such as HiFi Assembly and Gibson Assembly Ultra correct this problem. Basic Gibson Assembly can introduce errors at fragment junctions.Gibson Assembly Master Mixes are relatively expensive commercial products but are available from multiple sources.The difference in speed is magnified when using Gibson assembly to clone multiple fragments at one time. Finally, the technique is fast compared to traditional restriction enzyme cloning.Gibson assembly is a one-pot assembly technique for as many as 15 separate fragments. ![]()
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